5J7C
A picomolar affinity FN3 domain in complex with hen egg-white lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.858, 87.725, 100.895 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.510 - 2.535 |
R-factor | 0.2192 |
Rwork | 0.218 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fnf 4z98 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.562 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155:000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.510 | 2.626 |
High resolution limit [Å] | 2.535 | 2.535 |
Rmerge | 0.244 | 1.264 |
Number of reflections | 16719 | |
<I/σ(I)> | 8.9 | 2.13 |
Completeness [%] | 99.0 | 96 |
Redundancy | 6.7 | 6.5 |
CC(1/2) | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 10% PEG 6000, 0.1 M Bicine, pH 8.8 |