5IUK

Crystal structure of the DesK-DesR complex in the phosphotransfer state with high Mg2+ (150 mM)

5IUK の概要

• エントリーDOI: 10.2210/pdb5iuk/pdb
• 関連するPDBエントリー: 5IUJ 5IUL 5IUM 5IUN
• 分子名称: Sensor histidine kinase DesK, Transcriptional regulatory protein DesR, PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER, ... (6 entities in total)
• 機能のキーワード: two-component regulatory system, kinase, response regulator, phosphotransfer complex, phosphotransfer, transferase, transferase-gene regulation complex, transferase/gene regulation
• 由来する生物種: Bacillus subtilis; 詳細
• 細胞内の位置: Cell membrane ; Multi-pass membrane protein : O34757; Cytoplasm : O34723
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 132263.65

構造登録者

Trajtenberg, F.,Imelio, J.A.,Larrieux, N.,Buschiazzo, A. (登録日: 2016-03-18, 公開日: 2016-12-21, 最終更新日: 2024-03-06)

主引用文献

Trajtenberg, F.,Imelio, J.A.,Machado, M.R.,Larrieux, N.,Marti, M.A.,Obal, G.,Mechaly, A.E.,Buschiazzo, A.
Regulation of signaling directionality revealed by 3D snapshots of a kinase:regulator complex in action.
Elife, 5:-, 2016

PubMed Abstract: Two-component systems (TCS) are protein machineries that enable cells to respond to input signals. Histidine kinases (HK) are the sensory component, transferring information toward downstream response regulators (RR). HKs transfer phosphoryl groups to their specific RRs, but also dephosphorylate them, overall ensuring proper signaling. The mechanisms by which HKs discriminate between such disparate directions, are yet unknown. We now disclose crystal structures of the HK:RR complex DesK:DesR from , comprising snapshots of the phosphotransfer and the dephosphorylation reactions. The HK dictates the reactional outcome through conformational rearrangements that include the reactive histidine. The phosphotransfer center is asymmetric, poised for dissociative nucleophilic substitution. The structural bases of HK phosphatase/phosphotransferase control are uncovered, and the unexpected discovery of a dissociative reactional center, sheds light on the evolution of TCS phosphotransfer reversibility. Our findings should be applicable to a broad range of signaling systems and instrumental in synthetic TCS rewiring.

PubMed: 27938660
DOI: 10.7554/eLife.21422

実験手法

X-RAY DIFFRACTION (2.9 Å)