5IUK

Crystal structure of the DesK-DesR complex in the phosphotransfer state with high Mg2+ (150 mM)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, D, E)	Sensor histidine kinase DesK	polymer	218	24956.6	4	UniProt (O34757) Pfam (PF07730) Pfam (PF02518)	Bacillus subtilis
2	E, F (C, F)	Transcriptional regulatory protein DesR	polymer	139	15108.4	2	UniProt (O34723) Pfam (PF00072)	Bacillus subtilis (strain 168)
3	G, I, K, M (A, B, D, E)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	4	Chemie (ACP)
4	H, J, L, N, O (A, B, D, E, C)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
5	P, Q (C, F)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
6	R, S, T, U, V... (A, B, D, E, C...)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

A, B, D, E: 154 - 370 (UniProt: O34757)

PDB	External Database	Details
Gly 153	-	expression tag
Glu 188	His 188	engineered mutation

C, F: 1 - 135 (UniProt: O34723)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	130043.1
Non-Polymers*	Number of molecules	11
	Total formula weight	2220.6
All*	Total formula weight	132263.6

*Water molecules are not included.