5IUK
Crystal structure of the DesK-DesR complex in the phosphotransfer state with high Mg2+ (150 mM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.930, 115.610, 91.590 |
Unit cell angles | 90.00, 116.71, 90.00 |
Refinement procedure
Resolution | 39.270 - 2.900 |
R-factor | 0.198 |
Rwork | 0.195 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.250 |
Data scaling software | XDS (Jun 17, 2015) |
Phasing software | PHASER (2.5.7) |
Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.780 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.116 | 1.471 |
Number of reflections | 36132 | |
<I/σ(I)> | 12.5 | |
Completeness [%] | 99.1 | 99.2 |
Redundancy | 5.8 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG3350, tri-potassium citrate, vapor diffusion, hanging drop, temperature 291K |