Crystal structure of VRC01c-HuGL2 Fab from an HIV-1 naive donor in complex with with a germline-targeting gp120 engineered outer domain eOD-GT8 at 2.44 A

Summary for 5IF0

Related5IFA 5IES
DescriptorVRC01cHuGL2 Fab heavy chain, VRC01c-HuGL2 Fab light chain, Germline-targeting HIV-1 gp120 engineered outer domain eOD-GT8, ... (6 entities in total)
Functional Keywordshiv-1 gp120, engineered outer domain (eod), germline targeting, cd4 binding site, vrc01-class naive human germline antibody, immune system, viral-protein complex, immune system-viral protein complex, immune system/viral protein
Biological sourceHomo sapiens
Total number of polymer chains6
Total molecular weight135620.26
Sarkar, A.,Wilson, I.A. (deposition date: 2016-02-25, release date: 2016-04-06, Last modification date: 2020-07-29)
Primary citation
Jardine, J.G.,Kulp, D.W.,Havenar-Daughton, C.,Sarkar, A.,Briney, B.,Sok, D.,Sesterhenn, F.,Ereno-Orbea, J.,Kalyuzhniy, O.,Deresa, I.,Hu, X.,Spencer, S.,Jones, M.,Georgeson, E.,Adachi, Y.,Kubitz, M.,deCamp, A.C.,Julien, J.P.,Wilson, I.A.,Burton, D.R.,Crotty, S.,Schief, W.R.
HIV-1 broadly neutralizing antibody precursor B cells revealed by germline-targeting immunogen.
Science, 351:1458-1463, 2016
PubMed: 27013733 (PDB entries with the same primary citation)
DOI: 10.1126/science.aad9195
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2323 0.3% 2.1% 1.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
PDB entries from 2020-09-16