5HYP

Structure of human C4b-binding protein alpha cain CCP domains 1 and 2 in complex with the hypervariable region of group A Streptococcus M28 protein

5HYP の概要

• エントリーDOI: 10.2210/pdb5hyp/pdb
• 関連するPDBエントリー: 5HYT 5HYU 5HZP 5I0Q
• 分子名称: C4b-binding protein alpha chain, M28 protein (2 entities in total)
• 機能のキーワード: m protein, complement, streptococcus pyogenes, hypervariable antigen, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26357.32

構造登録者

Buffalo, C.Z.,Bahn-Suh, A.J.,Ghosh, P. (登録日: 2016-02-01, 公開日: 2016-07-20, 最終更新日: 2024-11-06)

主引用文献

Buffalo, C.Z.,Bahn-Suh, A.J.,Hirakis, S.P.,Biswas, T.,Amaro, R.E.,Nizet, V.,Ghosh, P.
Conserved patterns hidden within group A Streptococcus M protein hypervariability recognize human C4b-binding protein.
Nat Microbiol, 1:16155-16155, 2016

PubMed Abstract: No vaccine exists against group A Streptococcus (GAS), a leading cause of worldwide morbidity and mortality. A severe hurdle is the hypervariability of its major antigen, the M protein, with >200 different M types known. Neutralizing antibodies typically recognize M protein hypervariable regions (HVRs) and confer narrow protection. In stark contrast, human C4b-binding protein (C4BP), which is recruited to the GAS surface to block phagocytic killing, interacts with a remarkably large number of M protein HVRs (apparently ∼90%). Such broad recognition is rare, and we discovered a unique mechanism for this through the structure determination of four sequence-diverse M proteins in complexes with C4BP. The structures revealed a uniform and tolerant 'reading head' in C4BP, which detected conserved sequence patterns hidden within hypervariability. Our results open up possibilities for rational therapies that target the M-C4BP interaction, and also inform a path towards vaccine design.

PubMed: 27595425
DOI: 10.1038/nmicrobiol.2016.155

実験手法

X-RAY DIFFRACTION (3.024 Å)