5H5Q
Crystal structure of human GPX4 in complex with GXpep-1
Summary for 5H5Q
Entry DOI | 10.2210/pdb5h5q/pdb |
Related | 5H5R 5H5S |
Descriptor | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, GXpep-1, GLYCEROL, ... (4 entities in total) |
Functional Keywords | phospholipid hydroperoxide glutathione peroxidase 4, selenocysteine, oxidoreductase, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor |
Biological source | Homo sapiens (Human) More |
Cellular location | Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm: P36969 |
Total number of polymer chains | 2 |
Total formula weight | 21336.34 |
Authors | Sogabe, S.,Kadotani, A.,Lane, W.,Snell, G. (deposition date: 2016-11-09, release date: 2016-12-07, Last modification date: 2023-11-15) |
Primary citation | Sakamoto, K.,Sogabe, S.,Kamada, Y.,Matsumoto, S.I.,Kadotani, A.,Sakamoto, J.I.,Tani, A. Discovery of GPX4 inhibitory peptides from random peptide T7 phage display and subsequent structural analysis Biochem. Biophys. Res. Commun., 482:195-201, 2017 Cited by PubMed Abstract: The phospholipid hydroperoxidase glutathione peroxidase (GPX4) is an enzyme that reduces lipid hydroperoxides in lipid membranes. Recently, GPX4 has been investigated as a target molecule that induces iron-dependent cell death (ferroptosis) selectively in cancer cells that express mutant Ras. GPX4 inhibitors have the potential to become novel anti-cancer drugs. However, there are no druggable pockets for conventional small molecules on the molecular surface of GPX4. To generate GPX4 inhibitors, we examined the use of peptides as an alternative to small molecules. By screening peptide libraries displayed on T7 phages, and analyzing the X-ray crystal structures of the peptides, we successfully identified one peptide that binds to near Sec73 of catalytic site and two peptides that bind to another site on GPX4. To our knowledge, this is the first study reporting GPX4 inhibitory peptides and their structural information. PubMed: 27836545DOI: 10.1016/j.bbrc.2016.11.035 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.1 Å) |
Structure validation
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