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5H5Q

Crystal structure of human GPX4 in complex with GXpep-1

Summary for 5H5Q
Entry DOI10.2210/pdb5h5q/pdb
Related5H5R 5H5S
DescriptorPhospholipid hydroperoxide glutathione peroxidase, mitochondrial, GXpep-1, GLYCEROL, ... (4 entities in total)
Functional Keywordsphospholipid hydroperoxide glutathione peroxidase 4, selenocysteine, oxidoreductase, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
Biological sourceHomo sapiens (Human)
More
Cellular locationIsoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm: P36969
Total number of polymer chains2
Total formula weight21336.34
Authors
Sogabe, S.,Kadotani, A.,Lane, W.,Snell, G. (deposition date: 2016-11-09, release date: 2016-12-07, Last modification date: 2023-11-15)
Primary citationSakamoto, K.,Sogabe, S.,Kamada, Y.,Matsumoto, S.I.,Kadotani, A.,Sakamoto, J.I.,Tani, A.
Discovery of GPX4 inhibitory peptides from random peptide T7 phage display and subsequent structural analysis
Biochem. Biophys. Res. Commun., 482:195-201, 2017
Cited by
PubMed Abstract: The phospholipid hydroperoxidase glutathione peroxidase (GPX4) is an enzyme that reduces lipid hydroperoxides in lipid membranes. Recently, GPX4 has been investigated as a target molecule that induces iron-dependent cell death (ferroptosis) selectively in cancer cells that express mutant Ras. GPX4 inhibitors have the potential to become novel anti-cancer drugs. However, there are no druggable pockets for conventional small molecules on the molecular surface of GPX4. To generate GPX4 inhibitors, we examined the use of peptides as an alternative to small molecules. By screening peptide libraries displayed on T7 phages, and analyzing the X-ray crystal structures of the peptides, we successfully identified one peptide that binds to near Sec73 of catalytic site and two peptides that bind to another site on GPX4. To our knowledge, this is the first study reporting GPX4 inhibitory peptides and their structural information.
PubMed: 27836545
DOI: 10.1016/j.bbrc.2016.11.035
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.1 Å)
Structure validation

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