5H2H

A three dimensional movie of structural changes in bacteriorhodopsin: structure obtained 40 ns after photoexcitation

Summary for 5H2H

• Entry DOI: 10.2210/pdb5h2h/pdb
• Related: 5B6V 5B6W 5B6X 5B6Y 5B6Z 5H2I 5H2J 5H2K 5H2L 5H2M 5H2N 5H2O 5H2P
• Descriptor: Bacteriorhodopsin, nonane, TETRADECANE, ... (12 entities in total)
• Functional Keywords: bacteriorhodopsin, xfel, time-resolved serial femtosecond crystallography, sacla, proton transport
• Biological source: Halobacterium salinarum NRC-1 (Halobacterium sp. NRC-1)
• Cellular location: Cell membrane ; Multi-pass membrane protein : P02945
• Total number of polymer chains: 1
• Total formula weight: 30891.94

Authors

Royant, A.,Nango, E.,Nakane, T.,Tanaka, T.,Arima, T.,Neutze, R.,Iwata, S. (deposition date: 2016-10-15, release date: 2016-12-21, Last modification date: 2024-10-16)

Primary citation

Nango, E.,Royant, A.,Kubo, M.,Nakane, T.,Wickstrand, C.,Kimura, T.,Tanaka, T.,Tono, K.,Song, C.,Tanaka, R.,Arima, T.,Yamashita, A.,Kobayashi, J.,Hosaka, T.,Mizohata, E.,Nogly, P.,Sugahara, M.,Nam, D.,Nomura, T.,Shimamura, T.,Im, D.,Fujiwara, T.,Yamanaka, Y.,Jeon, B.,Nishizawa, T.,Oda, K.,Fukuda, M.,Andersson, R.,Bath, P.,Dods, R.,Davidsson, J.,Matsuoka, S.,Kawatake, S.,Murata, M.,Nureki, O.,Owada, S.,Kameshima, T.,Hatsui, T.,Joti, Y.,Schertler, G.,Yabashi, M.,Bondar, A.N.,Standfuss, J.,Neutze, R.,Iwata, S.
A three-dimensional movie of structural changes in bacteriorhodopsin
Science, 354:1552-1557, 2016

PubMed Abstract: Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.

PubMed: 28008064
DOI: 10.1126/science.aah3497

Experimental method

X-RAY DIFFRACTION (2.1 Å)