5H2M

A three dimensional movie of structural changes in bacteriorhodopsin: structure obtained 13.8 us after photoexcitation

Summary for 5H2M

Related5B6V 5B6W 5B6X 5B6Y 5B6Z 5H2H 5H2I 5H2J 5H2K 5H2L 5H2N 5H2O 5H2P
DescriptorBacteriorhodopsin, RETINAL, 2,3-DI-PHYTANYL-GLYCEROL, ... (12 entities in total)
Functional Keywordsbacteriorhodopsin, xfel, time-resolved serial femtosecond crystallography, sacla, proton transport
Biological sourceHalobacterium salinarum NRC-1 (Halobacterium sp. NRC-1)
Cellular locationCell membrane ; Multi-pass membrane protein  P02945
Total number of polymer chains1
Total molecular weight30891.94
Authors
Royant, A.,Nango, E.,Nakane, T.,Tanaka, T.,Arima, T.,Neutze, R.,Iwata, S. (deposition date: 2016-10-15, release date: 2016-12-21, Last modification date: 2018-01-31)
Primary citation
Nango, E.,Royant, A.,Kubo, M.,Nakane, T.,Wickstrand, C.,Kimura, T.,Tanaka, T.,Tono, K.,Song, C.,Tanaka, R.,Arima, T.,Yamashita, A.,Kobayashi, J.,Hosaka, T.,Mizohata, E.,Nogly, P.,Sugahara, M.,Nam, D.,Nomura, T.,Shimamura, T.,Im, D.,Fujiwara, T.,Yamanaka, Y.,Jeon, B.,Nishizawa, T.,Oda, K.,Fukuda, M.,Andersson, R.,Bath, P.,Dods, R.,Davidsson, J.,Matsuoka, S.,Kawatake, S.,Murata, M.,Nureki, O.,Owada, S.,Kameshima, T.,Hatsui, T.,Joti, Y.,Schertler, G.,Yabashi, M.,Bondar, A.N.,Standfuss, J.,Neutze, R.,Iwata, S.
A three-dimensional movie of structural changes in bacteriorhodopsin
Science, 354:1552-1557, 2016
PubMed: 28008064 (PDB entries with the same primary citation)
DOI: 10.1126/science.aah3497
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.1 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.18530.4%016.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5h2m
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Molmil generated image of 5h2m
rotated about x axis by 90°
Molmil generated image of 5h2m
rotated about y axis by 90°

More Biological unit images

Molmil generated image of 5h2m
no rotation
Molmil generated image of 5h2m
rotated about x axis by 90°
Molmil generated image of 5h2m
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (5h2m.pdb1.gz [294.89 KB])
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