5GID

Crystal structure of VDR in complex with DLAM-4 (C2 form)

5GID の概要

• エントリーDOI: 10.2210/pdb5gid/pdb
• 関連するPDBエントリー: 5GIC 5GIE
• 分子名称: Vitamin D3 receptor, SRC1, (3R,5S)-5-[(2R)-2-[(1R,3aS,4E,7aR)-7a-methyl-4-[(2Z)-2-[(3S,5R)-2-methylidene-3,5-bis(oxidanyl)cyclohexylidene]ethylidene]-2,3,3a,5,6,7-hexahydro-1H-inden-1-yl]propyl]-3-methyl-3-oxidanyl-1-(4-phenylbutyl)pyrrolidin-2-one, ... (4 entities in total)
• 機能のキーワード: nuclear receptor, transcription
• 由来する生物種: Rattus norvegicus (Rat); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29916.60

構造登録者

Asano, L.,Shimizu, T. (登録日: 2016-06-23, 公開日: 2016-12-07, 最終更新日: 2024-03-20)

主引用文献

Asano, L.,Waku, T.,Abe, R.,Kuwabara, N.,Ito, I.,Yanagisawa, J.,Nagasawa, K.,Shimizu, T.
Regulation of the vitamin D receptor by vitamin D lactam derivatives.
Febs Lett., 590:3270-3279, 2016

PubMed Abstract: The active metabolite of vitamin D3 , 1α,25-dihydroxyvitamin D3 , acts as a ligand for the vitamin D receptor (VDR) and activates VDR-mediated gene expression. Recently, we characterized 1α,25-dihydroxyvitamin D3 -26,23-lactams (DLAMs), which mimic vitamin D3 metabolites, as noncalcemic VDR ligands that barely activate the receptor. In this study, we present structural insights onto the regulation of VDR function by DLAMs. X-ray crystallographic analysis revealed that DLAMs induced a large conformational change in the loop region between helices H6 and H7 in the VDR ligand-binding domain. Our structural analysis suggests that targeting of the loop region may be a new mode of VDR regulation.

PubMed: 27500498
DOI: 10.1002/1873-3468.12348

実験手法

X-RAY DIFFRACTION (2.151 Å)