5GAK

Yeast 60S ribosomal subunit with A-site tRNA, P-site tRNA and eIF-5A

This is a large structure.

Summary for 5GAK

EMDB information3227
Descriptor25S rRNA, 60S ribosomal protein L23-A, 5S rRNA, ... (50 entities in total)
Functional Keywordsribosome, translation, hypusine
Biological sourceSaccharomyces cerevisiae (Baker's yeast)
Cellular locationCytoplasm  P0CX41 P04449 P04456 P05743 P0C2H6 P0CX27 P02406 P0CX25 P05747 P0CX45 P14120 P14126 P0C2H8 P10664 P38061 P26321 P05744 Q02326 P87262 P05737 P0CX84 P17076 P05745 P05738 P49166 P0C0W9 P49167 Q12690 P04650 P36105 P05748 P0CX86 P26784 P23301 P05740 P0CX49 P41805 P0CX82 P0CX23 Q02753 P05749
Ubiquitin: Cytoplasm . 60S ribosomal protein L40-A: Cytoplasm  P0CH08
Total number of polymer chains49
Total molecular weight2016966.03
Authors
Schmidt, C.,Becker, T. (deposition date: 2015-12-09, release date: 2016-02-24, Last modification date: 2017-08-02)
Primary citation
Schmidt, C.,Becker, T.,Heuer, A.,Braunger, K.,Shanmuganathan, V.,Pech, M.,Berninghausen, O.,Wilson, D.N.,Beckmann, R.
Structure of the hypusinylated eukaryotic translation factor eIF-5A bound to the ribosome.
Nucleic Acids Res., 44:1944-1951, 2016
PubMed: 26715760 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gkv1517
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
?

Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone240.7%0.2%0.44MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5gak
no rotation
Molmil generated image of 5gak
rotated about x axis by 90°
Molmil generated image of 5gak
rotated about y axis by 90°
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