5GAK
Yeast 60S ribosomal subunit with A-site tRNA, P-site tRNA and eIF-5A
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.88 |
| Software | Signature,RELION,EPU,CTFFIND,PHENIX |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
