5FUR

Structure of human TFIID-IIA bound to core promoter DNA

Summary for 5FUR

• Entry DOI: 10.2210/pdb5fur/pdb
• EMDB information: 3305
• Descriptor: TATA-BOX-BINDING PROTEIN, TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6, TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8, ... (11 entities in total)
• Functional Keywords: tfiid, tfiia, transcription, rna polymerase ii, general transcription factors, preinitiation complex, core promoter, dna binding
• Biological source: HOMO SAPIENS (HUMAN); More
• Cellular location: Nucleus : P20226 P49848 Q7Z7C8 P52655 P52655 P52657 P21675 Q15545 Q6P1X5
• Total number of polymer chains: 12
• Total formula weight: 688289.19

Authors

Louder, R.K.,He, Y.,Lopez-Blanco, J.R.,Fang, J.,Chacon, P.,Nogales, E. (deposition date: 2016-01-29, release date: 2016-04-06, Last modification date: 2024-11-13)

Primary citation

Louder, R.K.,He, Y.,Lopez-Blanco, J.R.,Fang, J.,Chacon, P.,Nogales, E.
Structure of Promoter-Bound TFIID and Model of Human Pre-Initiation Complex Assembly.
Nature, 531:604-, 2016

PubMed Abstract: The general transcription factor IID (TFIID) plays a central role in the initiation of RNA polymerase II (Pol II)-dependent transcription by nucleating pre-initiation complex (PIC) assembly at the core promoter. TFIID comprises the TATA-binding protein (TBP) and 13 TBP-associated factors (TAF1-13), which specifically interact with a variety of core promoter DNA sequences. Here we present the structure of human TFIID in complex with TFIIA and core promoter DNA, determined by single-particle cryo-electron microscopy at sub-nanometre resolution. All core promoter elements are contacted by subunits of TFIID, with TAF1 and TAF2 mediating major interactions with the downstream promoter. TFIIA bridges the TBP-TATA complex with lobe B of TFIID. We also present the cryo-electron microscopy reconstruction of a fully assembled human TAF-less PIC. Superposition of common elements between the two structures provides novel insights into the general role of TFIID in promoter recognition, PIC assembly, and transcription initiation.

PubMed: 27007846
DOI: 10.1038/NATURE17394

Experimental method

ELECTRON MICROSCOPY (8.5 Å)