5FCJ

Structure of the anisomycin-containing uL3 W255C mutant 80S yeast ribosome

This is a large structure.

Summary for 5FCJ

Descriptor18S ribosomal RNA, 40S ribosomal protein S0-A, 40S ribosomal protein S1-A, ... (87 entities in total)
Functional Keywordsribosome, ul3 mutant, anisomycin
Biological sourceSaccharomyces cerevisiae S288c
More
Cellular locationCytoplasm  P32905 P33442 P25443 P05750 P0CX35 P26783 P0CX37 P26786 P0CX39 O13516 P48589 P05756 P06367 Q01855 P0CX51 P02407 P0CX55 P07280 P38701 P0C0V8 P0C0W1 P0CX29 P0CX31 Q3E792 P39939 P35997 Q3E7X9 P41057 P0CX33 P38011 P0CX45 P14126 P10664 P26321 Q02326 P05737 P17076 P05738 P41805 P0C0W9 Q12690 P36105 P05748 P26784 P05740 P0CX49 P0CX82 P0CX23 Q02753 P05749 P0CX41 P04449 P04456 P05743 P0C2H6 P02406 P05747 P14120 P0C2H8 P38061 P05744 P87262 P0CX84 P05745 P49166 P49167 P04650 P0CX86 P0CX27 P0CX25 P05317
Ubiquitin: Cytoplasm . 40S ribosomal protein S31: Cytoplasm  P05759
Ubiquitin: Cytoplasm . 60S ribosomal protein L40-A: Cytoplasm  P0CH08
Total number of polymer chains162
Total molecular weight6565290.54
Authors
Mailliot, J.,Garreau de Loubresse, N.,Yusupova, G.,Dinman, J.D.,Yusupov, M. (deposition date: 2015-12-15, release date: 2016-05-11, Last modification date: 2017-10-11)
Primary citation
Mailliot, J.,Garreau de Loubresse, N.,Yusupova, G.,Meskauskas, A.,Dinman, J.D.,Yusupov, M.
Crystal Structures of the uL3 Mutant Ribosome: Illustration of the Importance of Ribosomal Proteins for Translation Efficiency.
J.Mol.Biol., 428:2195-2202, 2016
PubMed: 26906928 (PDB entries with the same primary citation)
DOI: 10.1016/j.jmb.2016.02.013
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.1 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.288145.2%16.4%4.9%0.46MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report