5FCJ

Structure of the anisomycin-containing uL3 W255C mutant 80S yeast ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.15873
Spacegroup name	P 1 21 1
Unit cell lengths	436.110, 287.310, 303.990
Unit cell angles	90.00, 98.86, 90.00

Refinement procedure

Resolution	49.958 - 3.100
R-factor	0.2356
Rwork	0.234
R-free	0.29060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v88
RMSD bond length	0.009
RMSD bond angle	1.221
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.958		3.200
High resolution limit [Å]	3.100	40.000	3.100
Rmerge	0.389	0.166	2.113
Rmeas	0.407	0.181	2.290
Total number of observations	12034750
Number of reflections	1333169	323	120881
<I/σ(I)>	6.77	6.35	0.93
Completeness [%]	100.0	47.5	100
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	Tris-Acetate pH7.0, KSCN, Mg-Acetate, Glycerol, Spermidine, PEG20000