5FCJ
Structure of the anisomycin-containing uL3 W255C mutant 80S yeast ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.15873 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 436.110, 287.310, 303.990 |
| Unit cell angles | 90.00, 98.86, 90.00 |
Refinement procedure
| Resolution | 49.958 - 3.100 |
| R-factor | 0.2356 |
| Rwork | 0.234 |
| R-free | 0.29060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v88 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.221 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.958 | 3.200 | |
| High resolution limit [Å] | 3.100 | 40.000 | 3.100 |
| Rmerge | 0.389 | 0.166 | 2.113 |
| Rmeas | 0.407 | 0.181 | 2.290 |
| Total number of observations | 12034750 | ||
| Number of reflections | 1333169 | 323 | 120881 |
| <I/σ(I)> | 6.77 | 6.35 | 0.93 |
| Completeness [%] | 100.0 | 47.5 | 100 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | Tris-Acetate pH7.0, KSCN, Mg-Acetate, Glycerol, Spermidine, PEG20000 |
