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5FCJ

Structure of the anisomycin-containing uL3 W255C mutant 80S yeast ribosome

This is a non-PDB format compatible entry.
Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, BC
(2, 6)
18S ribosomal RNApolymer1800579761.92REF (831416132)Saccharomyces cerevisiae S288c
2B, CC
(S0, s0)
40S ribosomal protein S0-Apolymer25127920.12UniProt (P32905)
Pfam (PF00318)
Saccharomyces cerevisiae S288c (Baker's yeast)Nucleic acid-binding protein NAB1A
3C, DC
(S1, s1)
40S ribosomal protein S1-Apolymer25428667.32UniProt (P33442)
Pfam (PF01015)
Saccharomyces cerevisiae S288c (Baker's yeast)RP10A
4D, EC
(S2, s2)
40S ribosomal protein S2polymer25327359.62UniProt (P25443)
Pfam (PF00333)
Pfam (PF03719)
Saccharomyces cerevisiae S288c (Baker's yeast)Omnipotent suppressor protein SUP44,RP12,S4,YS5
5E, FC
(S3, s3)
40S ribosomal protein S3polymer23926411.62UniProt (P05750)
Pfam (PF07650)
Pfam (PF00189)
Saccharomyces cerevisiae S288c (Baker's yeast)RP13,YS3
6F, GC
(S4, s4)
40S ribosomal protein S4-Apolymer26029338.12UniProt (P0CX35)
Pfam (PF08071)
Pfam (PF01479)
Pfam (PF00900)
Pfam (PF00467)
Pfam (PF16121)
Saccharomyces cerevisiae S288c (Baker's yeast)RP5,S7,YS6
7G, HC
(S5, s5)
40S ribosomal protein S5polymer22424941.42UniProt (P26783)
Pfam (PF00177)
Saccharomyces cerevisiae S288c (Baker's yeast)RP14,S2,YS8
8H, IC
(S6, s6)
40S ribosomal protein S6-Apolymer23627054.52UniProt (P0CX37)
Pfam (PF01092)
Saccharomyces cerevisiae S288c (Baker's yeast)RP9,S10,YS4
9I, JC
(S7, s7)
40S ribosomal protein S7-Apolymer18921527.02UniProt (P26786)
Pfam (PF01251)
Saccharomyces cerevisiae S288c (Baker's yeast)RP30,RP40
10J, KC
(S8, s8)
40S ribosomal protein S8-Apolymer20022537.82UniProt (P0CX39)
Pfam (PF01201)
Saccharomyces cerevisiae S288c (Baker's yeast)RP19,S14,YS9
11K, LC
(S9, s9)
40S ribosomal protein S9-Apolymer19622356.72UniProt (O13516)
Pfam (PF00163)
Pfam (PF01479)
Saccharomyces cerevisiae S288c (Baker's yeast)RP21,S13,YP28,YS11
12L, MC
(C0, c0)
40S ribosomal protein S10-A,40S ribosomal protein S10-A,40S ribosomal protein S10-A,40S ribosomal protein S10-Apolymer9611181.82UniProt (Q08745)
Pfam (PF03501)
Saccharomyces cerevisiae S288c (Baker's yeast)
13M, NC
(C1, c1)
40S ribosomal protein S11-A,40S ribosomal protein S11-A,40S ribosomal protein S11-A,40S ribosomal protein S11-A,40S ribosomal protein S11-A (uS17)polymer15517368.42UniProt (P0CX47)
Pfam (PF16205)
Pfam (PF00366)
Saccharomyces cerevisiae S288c (Baker's yeast)RP41,S18,YS12,RP41,S18,YS12,RP41,S18,YS12,RP41,S18,YS12
14N, OC
(C2, c2)
40S ribosomal protein S12polymer14215357.42UniProt (P48589)
Pfam (PF01248)
Saccharomyces cerevisiae S288c (Baker's yeast)
15O, PC
(C3, c3)
40S ribosomal protein S13polymer15016928.72UniProt (P05756)
Pfam (PF08069)
Pfam (PF00312)
Saccharomyces cerevisiae S288c (Baker's yeast)S27a,YS15
16P, QC
(C4, c4)
40S ribosomal protein S14-Apolymer13614431.52UniProt (P06367)
Pfam (PF00411)
Saccharomyces cerevisiae S288c (Baker's yeast)RP59A
17Q, RC
(C5, c5)
40S ribosomal protein S15polymer14115900.72UniProt (Q01855)
Pfam (PF00203)
Saccharomyces cerevisiae S288c (Baker's yeast)RIG protein,RP52,S21,YS21
18R, SC
(C6, c6)
40S ribosomal protein S16-Apolymer14215746.32UniProt (P0CX51)
Pfam (PF00380)
Saccharomyces cerevisiae S288c (Baker's yeast)RP61R
19S, TC
(C7, c7)
40S ribosomal protein S17-Apolymer13615820.42UniProt (P02407)
Pfam (PF00833)
Saccharomyces cerevisiae S288c (Baker's yeast)RP51A
20T, UC
(C8, c8)
40S ribosomal protein S18-Apolymer14516940.42UniProt (P0CX55)
Pfam (PF00416)
Saccharomyces cerevisiae S288c (Baker's yeast)
21U, VC
(C9, c9)
40S ribosomal protein S19-Apolymer14315810.92UniProt (P07280)
Pfam (PF01090)
Saccharomyces cerevisiae S288c (Baker's yeast)RP55A,S16a,YP45,YS16A
22V, WC
(D0, d0)
40S ribosomal protein S20polymer12013797.92UniProt (P38701)
Pfam (PF00338)
Saccharomyces cerevisiae S288c (Baker's yeast)
23W, XC
(D1, d1)
40S ribosomal protein S21-Apolymer879758.82UniProt (P0C0V8)
Pfam (PF01249)
Saccharomyces cerevisiae S288c (Baker's yeast)S26,YS25
24X, YC
(D2, d2)
40S ribosomal protein S22-Apolymer12914518.92UniProt (P0C0W1)
Pfam (PF00410)
Saccharomyces cerevisiae S288c (Baker's yeast)RP50,S24,YP58,YS22
25Y, ZC
(D3, d3)
40S ribosomal protein S23-Apolymer14415942.72UniProt (P0CX29)
Pfam (PF00164)
Saccharomyces cerevisiae S288c (Baker's yeast)RP37,S28,YS14
26AD, Z
(D4, d4)
40S ribosomal protein S24-Apolymer13415231.62UniProt (P0CX31)
Pfam (PF01282)
Saccharomyces cerevisiae S288c (Baker's yeast)RP50
27AA, BD
(D5, d5)
40S ribosomal protein S25-Apolymer10711936.12UniProt (Q3E792)
Pfam (PF03297)
Saccharomyces cerevisiae S288c (Baker's yeast)RP45,S31,YS23
28BA, CD
(D6, d6)
40S ribosomal protein S26-Bpolymer9711023.02UniProt (P39939)
Pfam (PF01283)
Saccharomyces cerevisiae S288c (Baker's yeast)
29CA, DD
(D7, d7)
40S ribosomal protein S27-Apolymer818762.22UniProt (P35997)
Pfam (PF01667)
Saccharomyces cerevisiae S288c (Baker's yeast)RP61,YS20
30DA, ED
(D8, d8)
40S ribosomal protein S28-Apolymer667474.72UniProt (Q3E7X9)
Pfam (PF01200)
Saccharomyces cerevisiae S288c (Baker's yeast)S33,YS27
31EA, FD
(D9, d9)
40S ribosomal protein S29-Apolymer556544.52UniProt (P41057)
Pfam (PF00253)
Saccharomyces cerevisiae S288c (Baker's yeast)S36,YS29
32FA, GD
(E0, e0)
40S ribosomal protein S30-Apolymer627006.32UniProt (P0CX33)
Pfam (PF04758)
Saccharomyces cerevisiae S288c (Baker's yeast)
33GA, HD
(E1, e1)
Ubiquitin-40S ribosomal protein S31polymer768703.52UniProt (P05759)
Pfam (PF01599)
Saccharomyces cerevisiae S288c (Baker's yeast)
34HA, ID
(SR, sR)
Guanine nucleotide-binding protein subunit beta-like proteinpolymer31834710.02UniProt (P38011)
Pfam (PF00400)
Saccharomyces cerevisiae S288c (Baker's yeast)Receptor for activated C kinase,Receptor of activated protein kinase C 1,RACK1
35IA, JD
(SM, sM)
Suppressor protein STM1,Suppressor protein STM1,Suppressor protein STM1,Suppressor protein STM1,Suppressor protein STM1polymer18218715.92UniProt (P39015)
Pfam (PF09598)
Saccharomyces cerevisiae S288c (Baker's yeast)3BP1,GU4 nucleic-binding protein 2,G4p2 protein,POP2 multicopy suppressor protein 4,Ribosomal subunits association factor,AF,TOM1 suppressor protein 1,Triplex-binding protein 1,3BP1,GU4 nucleic-binding protein 2,G4p2 protein,POP2 multicopy suppressor protein 4,Ribosomal subunits association factor,AF,TOM1 suppressor protein 1,Triplex-binding protein 1,3BP1,GU4 nucleic-binding protein 2,G4p2 protein,POP2 multicopy suppressor protein 4,Ribosomal subunits association factor,AF,TOM1 suppressor protein 1,Triplex-binding protein 1,3BP1,GU4 nucleic-binding protein 2,G4p2 protein,POP2 multicopy suppressor protein 4,Ribosomal subunits association factor,AF,TOM1 suppressor protein 1,Triplex-binding protein 1
36JA, KD
(1, 5)
25S ribosomal RNApolymer33961097493.92REF (831416132)Saccharomyces cerevisiae S288c
37KA, LD
(3, 7)
5S ribosomal RNApolymer12138951.12GenBank (834774822)Saccharomyces cerevisiae S288c
38LA, MD
(4, 8)
5.8S ribosomal RNApolymer15850682.92GenBank (940534893)Saccharomyces cerevisiae S288c
39MA, ND
(L2, l2)
60S ribosomal protein L2-Apolymer25327332.42UniProt (P0CX45)
Pfam (PF00181)
Pfam (PF03947)
Saccharomyces cerevisiae S288c (Baker's yeast)L5,RP8,YL6
40NA, OD
(L3, l3)
60S ribosomal protein L3polymer38643636.52UniProt (P14126)
Pfam (PF00297)
Saccharomyces cerevisiae S288c (Baker's yeast)Maintenance of killer protein 8,RP1,Trichodermin resistance protein,YL1
41OA, PD
(L4, l4)
60S ribosomal protein L4-Apolymer36139027.92UniProt (P10664)
Pfam (PF00573)
Pfam (PF14374)
Saccharomyces cerevisiae S288c (Baker's yeast)L2,RP2,YL2
42PA, QD
(L5, l5)
60S ribosomal protein L5polymer29633633.62UniProt (P26321)
Pfam (PF17144)
Pfam (PF14204)
Saccharomyces cerevisiae S288c (Baker's yeast)L1,L1a,Ribosomal 5S RNA-binding protein,YL3
43QA, RD
(L6, l6)
60S ribosomal protein L6-Apolymer17519869.42UniProt (Q02326)
Pfam (PF01159)
Saccharomyces cerevisiae S288c (Baker's yeast)L17,RP18,YL16
44RA, SD
(L7, l7)
60S ribosomal protein L7-Apolymer24327555.12UniProt (P05737)
Pfam (PF08079)
Pfam (PF00327)
Saccharomyces cerevisiae S288c (Baker's yeast)L6,RP11,YL8
45SA, TD
(L8, l8)
60S ribosomal protein L8-Apolymer25528044.62UniProt (P17076)
Pfam (PF01248)
Saccharomyces cerevisiae S288c (Baker's yeast)L4,L4-2,L7a-1,Maintenance of killer protein 7,RP6,YL5
46TA, UD
(L9, l9)
60S ribosomal protein L9-Apolymer19121605.12UniProt (P05738)
Pfam (PF00347)
Saccharomyces cerevisiae S288c (Baker's yeast)L8,RP24,YL11
47UA, VD
(M0, m0)
60S ribosomal protein L10polymer22025279.32UniProt (P41805)
Pfam (PF00252)
Saccharomyces cerevisiae S288c (Baker's yeast)L9,Ubiquinol-cytochrome C reductase complex subunit VI-requiring protein
48VA, WD
(M1, m1)
60S ribosomal protein L11-Apolymer17319624.52UniProt (P0C0W9)
Pfam (PF00281)
Pfam (PF00673)
Saccharomyces cerevisiae S288c (Baker's yeast)L16,RP39,YL22
49WA, YD
(M3, m3)
60S ribosomal protein L13-Apolymer19822473.02UniProt (Q12690)
Pfam (PF01294)
Saccharomyces cerevisiae S288c (Baker's yeast)
50XA, ZD
(M4, m4)
60S ribosomal protein L14-Apolymer13715063.92UniProt (P36105)
Pfam (PF00467)
Pfam (PF01929)
Saccharomyces cerevisiae S288c (Baker's yeast)
51AE, YA
(M5, m5)
60S ribosomal protein L15-Apolymer20324351.22UniProt (P05748)
Pfam (PF00827)
Saccharomyces cerevisiae S288c (Baker's yeast)L13,RP15R,YL10,YP18
52BE, ZA
(M6, m6)
60S ribosomal protein L16-Apolymer19822116.02UniProt (P26784)
Pfam (PF00572)
Saccharomyces cerevisiae S288c (Baker's yeast)L13a,L21,RP22,YL15
53AB, CE
(M7, m7)
60S ribosomal protein L17-Apolymer18320458.32UniProt (P05740)
Pfam (PF00237)
Saccharomyces cerevisiae S288c (Baker's yeast)L20A,YL17
54BB, DE
(M8, m8)
60S ribosomal protein L18-Apolymer18520478.12UniProt (P0CX49)
Pfam (PF17135)
Saccharomyces cerevisiae S288c (Baker's yeast)RP28
55CB, EE
(M9, m9)
60S ribosomal protein L19-Apolymer18821631.12UniProt (P0CX82)
Pfam (PF01280)
Saccharomyces cerevisiae S288c (Baker's yeast)L23,RP15L,RP33,YL14
56DB, FE
(N0, n0)
60S ribosomal protein L20-Apolymer17220478.92UniProt (P0CX23)
Pfam (PF01775)
Saccharomyces cerevisiae S288c (Baker's yeast)L18a
57EB, GE
(N1, n1)
60S ribosomal protein L21-Apolymer15918148.12UniProt (Q02753)
Pfam (PF01157)
Saccharomyces cerevisiae S288c (Baker's yeast)
58FB, HE
(N2, n2)
60S ribosomal protein L22-Apolymer12013580.22UniProt (P05749)
Pfam (PF01776)
Saccharomyces cerevisiae S288c (Baker's yeast)L1c,RP4,YL31
59GB, IE
(N3, n3)
60S ribosomal protein L23-Apolymer13614362.82UniProt (P0CX41)
Pfam (PF00238)
Saccharomyces cerevisiae S288c (Baker's yeast)L17a,YL32
60HB, JE
(N4, n4)
60S ribosomal protein L24-Apolymer15517661.72UniProt (P04449)
Pfam (PF01246)
Saccharomyces cerevisiae S288c (Baker's yeast)L30,RP29,YL21
61IB, KE
(N5, n5)
60S ribosomal protein L25polymer14115656.42UniProt (P04456)
Pfam (PF03939)
Pfam (PF00276)
Saccharomyces cerevisiae S288c (Baker's yeast)RP16L,YL25,YP42'
62JB, LE
(N6, n6)
60S ribosomal protein L26-Apolymer12614134.62UniProt (P05743)
Pfam (PF16906)
Pfam (PF00467)
Saccharomyces cerevisiae S288c (Baker's yeast)L33,YL33
63KB, ME
(N7, n7)
60S ribosomal protein L27-Apolymer13515437.22UniProt (P0C2H6)
Pfam (PF01777)
Saccharomyces cerevisiae S288c (Baker's yeast)
64LB, NE
(N8, n8)
60S ribosomal protein L28polymer14816630.52UniProt (P02406)
Pfam (PF00828)
Saccharomyces cerevisiae S288c (Baker's yeast)L27a,L29,RP44,RP62,YL24
65MB, OE
(N9, n9)
60S ribosomal protein L29polymer586560.72UniProt (P05747)
Pfam (PF01779)
Saccharomyces cerevisiae S288c (Baker's yeast)YL43
66NB, PE
(O0, o0)
60S ribosomal protein L30polymer10411299.22UniProt (P14120)
Pfam (PF01248)
Saccharomyces cerevisiae S288c (Baker's yeast)L32,RP73,YL38
67OB, QE
(O1, o1)
60S ribosomal protein L31-Apolymer11212849.02UniProt (P0C2H8)
Pfam (PF01198)
Saccharomyces cerevisiae S288c (Baker's yeast)L34,YL28
68PB, RE
(O2, o2)
60S ribosomal protein L32polymer12914678.22UniProt (P38061)
Pfam (PF01655)
Saccharomyces cerevisiae S288c (Baker's yeast)
69QB, SE
(O3, o3)
60S ribosomal protein L33-Apolymer10612045.92UniProt (P05744)
Pfam (PF01247)
Saccharomyces cerevisiae S288c (Baker's yeast)L37,RP47,YL37
70RB, TE
(O4, o4)
60S ribosomal protein L34-Apolymer12013542.02UniProt (P87262)
Pfam (PF01199)
Saccharomyces cerevisiae S288c (Baker's yeast)
71SB, UE
(O5, o5)
60S ribosomal protein L35-Apolymer11913811.42UniProt (P0CX84)
Pfam (PF00831)
Saccharomyces cerevisiae S288c (Baker's yeast)
72TB, VE
(O6, o6)
60S ribosomal protein L36-Apolymer9911020.12UniProt (P05745)
Pfam (PF01158)
Saccharomyces cerevisiae S288c (Baker's yeast)L39,YL39
73UB, WE
(O7, o7)
60S ribosomal protein L37-Apolymer879746.22UniProt (P49166)
Pfam (PF01907)
Saccharomyces cerevisiae S288c (Baker's yeast)L43,YL35,YP55
74VB, XE
(O8, o8)
60S ribosomal protein L38polymer778714.42UniProt (P49167)
Pfam (PF01781)
Saccharomyces cerevisiae S288c (Baker's yeast)
75WB, YE
(O9, o9)
60S ribosomal protein L39polymer506227.42UniProt (P04650)
Pfam (PF00832)
Saccharomyces cerevisiae S288c (Baker's yeast)L46,YL40
76XB, ZE
(Q0, q0)
Ubiquitin-60S ribosomal protein L40polymer526032.32UniProt (P0CH08)
Pfam (PF01020)
Saccharomyces cerevisiae S288c (Baker's yeast)
77AF, YB
(Q1, q1)
60S ribosomal protein L41-Apolymer253354.22UniProt (P0CX86)
Pfam (PF05162)
Saccharomyces cerevisiae S288c (Baker's yeast)L47,YL41
78BF, ZB
(Q2, q2)
60S ribosomal protein L42-Apolymer10512115.52UniProt (P0CX27)
Pfam (PF00935)
Saccharomyces cerevisiae S288c (Baker's yeast)L41,YL27,YP44
79AC, CF
(Q3, q3)
60S ribosomal protein L43-Apolymer919981.82UniProt (P0CX25)
Pfam (PF01780)
Saccharomyces cerevisiae S288c (Baker's yeast)L37a,YL35
80XD
(m2)
60S ribosomal protein L12-A (uL11)polymer15012783.81Saccharomyces cerevisiae S288c
81DF
(p0)
60S acidic ribosomal protein P0polymer31133617.91UniProt (P05317)
Pfam (PF00466)
Pfam (PF17777)
Pfam (PF00428)
Saccharomyces cerevisiae S288c (Baker's yeast)A0,L10E
82EF
(p1)
60S ribosomal protein P1 alphapolymer474017.91Saccharomyces cerevisiae S288c
83FF
(p2)
60S ribosomal protein P2 betapolymer463932.81Saccharomyces cerevisiae S288c
84AAA, ABC, ACB, ADB, AEB...
(1, 5, m5, 2, 3...)
MAGNESIUM IONnon-polymer24.3979Chemie (MG)
85AAB, AAC, ABA, ACA, ACC...
(6, 5, 1, 7, 8...)
osmium (III) hexamminenon-polymer286.41034Chemie (OHX)
86ABB, BBB, CBB, CFC, CO...
(d7, d9, e1, q0, E1...)
ZINC IONnon-polymer65.416Chemie (ZN)
87FO
(1)
ANISOMYCINnon-polymer265.31Chemie (ANM)
Sequence modifications
C0, c0: 1 - 84 (UniProt: Q08745)
C0, c0: 85 - 96 (PDB: 5FCJ)
C1, c1: 2 - 146 (UniProt: P0CX47)
C1, c1: 147 - 156 (PDB: 5FCJ)
SM, sM: 1 - 141 (UniProt: P39015)
SM, sM: 151 - 191 (PDB: 5FCJ)
L3, l3: 2 - 387 (UniProt: P14126)
PDBExternal DatabaseDetails
Cys 255Trp 255engineered mutation
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains162
Total formula weight6244082.7
Non-Polymers*Number of molecules2030
Total formula weight321207.9
All*Total formula weight6565290.5
*Water molecules are not included.

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PDB entries from 2024-10-30

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