5F8A

Crystal structure of the ternary EcoRV-DNA-Lu complex with uncleaved DNA substrate. Lanthanide binding to EcoRV-DNA complex inhibits cleavage.

Summary for 5F8A

• Entry DOI: 10.2210/pdb5f8a/pdb
• Related: 4ORJ 5HLK
• Descriptor: DNA (5'-D(*AP*AP*AP*GP*AP*TP*AP*TP*CP*TP*TP*T)-3'), Type-2 restriction enzyme EcoRV, LUTETIUM (III) ION, ... (6 entities in total)
• Functional Keywords: hydrolase, protein-dna complex, lutetium, hydrolase-dna complex, hydrolase/dna
• Biological source: Escherichia coli; More
• Total number of polymer chains: 4
• Total formula weight: 64959.22

Authors

Sangani, S.S.,Kehr, A.D.,Sinha, K.,Rule, G.S.,Jen-Jacobson, L. (deposition date: 2015-12-09, release date: 2016-11-09, Last modification date: 2023-09-27)

• Primary citation: Sinha, K.,Sangani, S.S.,Kehr, A.D.,Rule, G.S.,Jen-Jacobson, L.; Metal Ion Binding at the Catalytic Site Induces Widely Distributed Changes in a Sequence Specific Protein-DNA Complex.; Biochemistry, 55:6115-6132, 2016; PubMed: 27786446; DOI: 10.1021/acs.biochem.6b00919

Experimental method

X-RAY DIFFRACTION (1.76 Å)