5EMF

Crystal structure of RNA r(GCUGCUGC) with antisense PNA p(GCAGCAGC)

Summary for 5EMF

• Entry DOI: 10.2210/pdb5emf/pdb
• Related: 5EME
• Descriptor: RNA (5'-R(*GP*CP*UP*GP*CP*UP*GP*C)-3'), antisense PNA p(GCAGCAGC), CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: cug repeats, rna/pna duplex, antisense pna, myotonic dystrophy type 1, rna
• Biological source: Homo sapiens; More
• Total number of polymer chains: 2
• Total formula weight: 4793.58

Authors

Kiliszek, A.,Banaszak, K.,Dauter, Z.,Rypniewski, W. (deposition date: 2015-11-06, release date: 2016-01-13, Last modification date: 2024-11-20)

Primary citation

Kiliszek, A.,Banaszak, K.,Dauter, Z.,Rypniewski, W.
The first crystal structures of RNA-PNA duplexes and a PNA-PNA duplex containing mismatches-toward anti-sense therapy against TREDs.
Nucleic Acids Res., 44:1937-1943, 2016

PubMed Abstract: PNA is a promising molecule for antisense therapy of trinucleotide repeat disorders. We present the first crystal structures of RNA-PNA duplexes. They contain CUG repeats, relevant to myotonic dystrophy type I, and CAG repeats associated with poly-glutamine diseases. We also report the first PNA-PNA duplex containing mismatches. A comparison of the PNA homoduplex and the PNA-RNA heteroduplexes reveals PNA's intrinsic structural properties, shedding light on its reported sequence selectivity or intolerance of mismatches when it interacts with nucleic acids. PNA has a much lower helical twist than RNA and the resulting duplex has an intermediate conformation. PNA retains its overall conformation while locally there is much disorder, especially peptide bond flipping. In addition to the Watson-Crick pairing, the structures contain interesting interactions between the RNA's phosphate groups and the Π electrons of the peptide bonds in PNA.

PubMed: 26717983
DOI: 10.1093/nar/gkv1513

Experimental method

X-RAY DIFFRACTION (1.14 Å)