5D8B

Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch

This is a non-PDB format compatible entry.

Summary for 5D8B

• Entry DOI: 10.2210/pdb5d8b/pdb
• Descriptor: 50S ribosomal protein L2, 50S ribosomal protein L15, 50S ribosomal protein L16, ... (56 entities in total)
• Functional Keywords: non-aug initiation, wobble mismatch, cytosine-cytosine mismatch, ribosome, translation
• Biological source: Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039); More
• Total number of polymer chains: 110
• Total formula weight: 4505462.70

Authors

Svidritskiy, E.,Korostelev, A.A. (deposition date: 2015-08-17, release date: 2015-10-14, Last modification date: 2023-09-27)

Primary citation

Svidritskiy, E.,Korostelev, A.A.
Ribosome Structure Reveals Preservation of Active Sites in the Presence of a P-Site Wobble Mismatch.
Structure, 23:2155-2161, 2015

PubMed Abstract: Translation initiation in the P site occasionally occurs at atypical (non-AUG) start codons, including those forming a mismatch in the third (wobble) position. During elongation, however, a pyrimidine-pyrimidine wobble mismatch may trigger a translation quality-control mechanism, whereby the P-site mismatch is thought to perturb the downstream A-site codon or the decoding center, thereby reducing translation fidelity and inducing termination of aberrant translation. We report a crystal structure of the 70S initiation complex containing an AUC codon in the ribosomal P site. Remarkably, the ribosome stabilizes the mismatched codon-anticodon helix, arranging a normally disruptive cytosine-cytosine pair into a Watson-Crick-like conformation. Translation-competent conformations of the tRNA, mRNA, and decoding center suggest that a P-site wobble-position mismatch in the 70S initiation complex does not pre-arrange the mRNA or decoding center to favor subsequent miscoding events.

PubMed: 26412335
DOI: 10.1016/j.str.2015.08.011

Experimental method

X-RAY DIFFRACTION (3.63 Å)