5D8B

Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	211.980, 453.750, 620.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 3.630
Rwork	0.268
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v9q
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.9-1692)
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	60.000
High resolution limit [Å]	3.630
Number of reflections	661200
<I/σ(I)>	5.7
Completeness [%]	99.3
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex