5D7V

Crystal structure of PTK6 kinase domain

Summary for 5D7V

• Entry DOI: 10.2210/pdb5d7v/pdb
• Descriptor: Protein-tyrosine kinase 6, PHOSPHATE ION, GLYCEROL, ... (4 entities in total)
• Functional Keywords: transferase, kinase, brk, apo
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm: Q13882
• Total number of polymer chains: 4
• Total formula weight: 126048.65

Authors

Thakur, M.K.,Birudukota, S.,Swaminathan, S.,Tyagi, R.,Gosu, R. (deposition date: 2015-08-14, release date: 2016-08-17, Last modification date: 2024-11-13)

Primary citation

Thakur, M.K.,Kumar, A.,Birudukota, S.,Swaminathan, S.,Tyagi, R.,Gosu, R.
Crystal structure of the kinase domain of human protein tyrosine kinase 6 (PTK6) at 2.33 angstrom resolution
Biochem.Biophys.Res.Commun., 478:637-642, 2016

PubMed Abstract: Human Protein tyrosine kinase 6 (PTK6) (EC:2.7.10.2), also known as the breast tumor kinase (BRK), is an intracellular non-receptor Src-related tyrosine kinase expressed in a majority of human breast tumors and breast cancer cell lines, but its expression is low or completely absent in normal mammary glands. In the recent past, several studies have suggested that PTK6 is a potential therapeutic target in cancer. To understand its structural and functional properties, the PTK6 kinase domain (PTK6-KD) gene was cloned, overexpressed in a baculo-insect cell system, purified and crystallized at room temperature. X-ray diffraction data to 2.33 Å resolution was collected on a single PTK6-KD crystal, which belonged to the triclinic space group P1. The Matthews coefficient calculation suggested the presence of four protein molecules per asymmetric unit, with a solvent content of ∼50%.The structure has been solved by molecular replacement and crystal structure data submitted to the protein data bank under the accession number 5D7V. This is the first report of apo PTK6-KD structure crystallized in DFG-in and αC-helix-out conformation.

PubMed: 27480927
DOI: 10.1016/j.bbrc.2016.07.121

Experimental method

X-RAY DIFFRACTION (2.33 Å)