5CBN

Fusion protein of mbp3-16 and B4 domain of protein A from staphylococcal aureus with chemical cross-linker EY-CBS

Summary for 5CBN

• Entry DOI: 10.2210/pdb5cbn/pdb
• Related: 5CBO
• Descriptor: Maltose-binding periplasmic protein, mbp3-16,Immunoglobulin G-binding protein A, 2,2'-ethyne-1,2-diylbis{5-[(chloroacetyl)amino]benzenesulfonic acid}, ... (4 entities in total)
• Functional Keywords: fusion, ey-cbs, alpha helix, cross-linker, protein binding
• Biological source: Escherichia coli (strain K12); More
• Cellular location: Periplasm: P0AEX9; Secreted, cell wall ; Peptidoglycan-anchor : P38507
• Total number of polymer chains: 2
• Total formula weight: 60208.73

Authors

Jeong, W.H.,Lee, H.,Song, D.H.,Lee, J.O. (deposition date: 2015-07-01, release date: 2016-03-30, Last modification date: 2023-11-08)

• Primary citation: Jeong, W.H.,Lee, H.,Song, D.H.,Eom, J.H.,Kim, S.C.,Lee, H.S.,Lee, H.,Lee, J.O.; Connecting two proteins using a fusion alpha helix stabilized by a chemical cross linker.; Nat Commun, 7:11031-11031, 2016; PubMed: 26980593; DOI: 10.1038/ncomms11031

Experimental method

X-RAY DIFFRACTION (2.3 Å)