5CBN
Fusion protein of mbp3-16 and B4 domain of protein A from staphylococcal aureus with chemical cross-linker EY-CBS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-05-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.282, 75.827, 111.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.085 - 2.300 |
| R-factor | 0.1982 |
| Rwork | 0.193 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1svx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.063 | 0.024 | 0.505 |
| Total number of observations | 167724 | ||
| Number of reflections | 24863 | ||
| <I/σ(I)> | 15.5 | ||
| Completeness [%] | 99.7 | 99.9 | 99.2 |
| Redundancy | 6.7 | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 277 | 36% w/v PEG 2000, 0.2M magnesium chloride hexahydrate |
