5CBN

Fusion protein of mbp3-16 and B4 domain of protein A from staphylococcal aureus with chemical cross-linker EY-CBS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Maltose-binding periplasmic protein	polymer	369	40572.0	1	UniProt (P0AEX9) Pfam (PF01547) In PDB	Escherichia coli (strain K12)	MBP,MMBP,Maltodextrin-binding protein
2	A	mbp3-16,Immunoglobulin G-binding protein A	polymer	176	19115.4	1	UniProt (P38507) Pfam (PF02216) In PDB	synthetic construct	IgG-binding protein A,Staphylococcal protein A
3	A	2,2'-ethyne-1,2-diylbis{5-[(chloroacetyl)amino]benzenesulfonic acid}	non-polymer		521.3	1	Chemie (EYC)
4		water	water		18.0	113	Chemie (HOH)

Sequence modifications

B: 1 - 366 (UniProt: P0AEX9)

PDB	External Database	Details
Met -2	-	expression tag
Gly -1	-	expression tag
Met 0	-	expression tag

A: 12 - 135 (PDB: 5CBN)
A: 1218 - 1269 (UniProt: P38507)

PDB	External Database	Details
Ala 1219	Glu 219	engineered mutation
Ala 1222	Asn 222	engineered mutation
Cys 1226	Glu 226	engineered mutation
Ala 1240	Gly 240	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59687.4
Non-Polymers*	Number of molecules	1
	Total formula weight	521.3
All*	Total formula weight	60208.7

*Water molecules are not included.