5C4V

Ski-like protein

Summary for 5C4V

• Entry DOI: 10.2210/pdb5c4v/pdb
• Descriptor: Mothers against decapentaplegic homolog 4, Ski-like protein, GLYCEROL, ... (6 entities in total)
• Functional Keywords: complex, signaling protein
• Biological source: Homo sapiens (Human); More
• Cellular location: Cytoplasm : Q13485
• Total number of polymer chains: 6
• Total formula weight: 129141.40

Authors

Wallden, K.,Nyman, T.,Hallberg, B.M. (deposition date: 2015-06-18, release date: 2016-10-12, Last modification date: 2024-11-20)

Primary citation

Wallden, K.,Nyman, T.,Hallberg, B.M.
SnoN Stabilizes the SMAD3/SMAD4 Protein Complex.
Sci Rep, 7:46370-46370, 2017

PubMed Abstract: TGF-β signaling regulates cellular processes such as proliferation, differentiation and apoptosis through activation of SMAD transcription factors that are in turn modulated by members of the Ski-SnoN family. In this process, Ski has been shown to negatively modulate TGF-β signaling by disrupting active R-SMAD/Co-SMAD heteromers. Here, we show that the related regulator SnoN forms a stable complex with the R-SMAD (SMAD3) and the Co-SMAD (SMAD4). To rationalize this stabilization at the molecular level, we determined the crystal structure of a complex between the SAND domain of SnoN and the MH2-domain of SMAD4. This structure shows a binding mode that is compatible with simultaneous coordination of R-SMADs. Our results show that SnoN, and SMAD heteromers can form a joint structural core for the binding of other transcription modulators. The results are of fundamental importance for our understanding of the molecular mechanisms behind the modulation of TGF-β signaling.

PubMed: 28397834
DOI: 10.1038/srep46370

Experimental method

X-RAY DIFFRACTION (2.6 Å)