5C4V
Ski-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 213.540, 122.830, 51.570 |
| Unit cell angles | 90.00, 90.72, 90.00 |
Refinement procedure
| Resolution | 40.300 - 2.600 |
| R-factor | 0.20923 |
| Rwork | 0.208 |
| R-free | 0.24212 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.847 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.300 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 39718 | |
| <I/σ(I)> | 3.7 | 0.7 |
| Completeness [%] | 97.2 | 95.5 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 2.8-3.3 M sodium chloride, 0.1 M Bis-Tris pH 5.5 |
