Crystal structure of a transcribing RNA Polymerase II complex reveals a complete transcription bubble

Summary for 5C44

Related5C4A 5C4X 5C3E 5C4J
DescriptorDNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB3, ... (15 entities in total)
Functional Keywordsprotein-dna complex, rna polyermase ii, transcribing complex, transferase-dna-rna complex, transferase/dna/rna
Biological sourceSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Cellular locationNucleus P04050 P08518 P16370 P20433 P20434 P34087 P20436 P38902
Cytoplasm  P20435
Nucleus, nucleolus  P27999 P22139 P40422
Total number of polymer chains15
Total molecular weight550516.87
Barnes, C.O.,Calero, M.,Malik, I.,Spahr, H.,Zhang, Q.,Pullara, F.,Kaplan, C.D.,Calero, G. (deposition date: 2015-06-17, release date: 2015-08-26)
Primary citation
Barnes, C.O.,Calero, M.,Malik, I.,Graham, B.W.,Spahr, H.,Lin, G.,Cohen, A.E.,Brown, I.S.,Zhang, Q.,Pullara, F.,Trakselis, M.A.,Kaplan, C.D.,Calero, G.
Crystal Structure of a Transcribing RNA Polymerase II Complex Reveals a Complete Transcription Bubble.
Mol.Cell, 59:258-269, 2015
PubMed: 26186291 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2015.06.034
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRNA backbone0.29253.9%5.9%0.45MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
PDB entries from 2020-09-16