Crystal structure of a transcribing RNA Polymerase II complex reveals a complete transcription bubble

Summary for 5C4A

Related5C3E 5C44 5C4J 5C4X
DescriptorDNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB3, ... (17 entities in total)
Functional Keywordsprotein-dna complex, rna polymerase ii, transcribing complex, transcription bubble, transferase-rna-dna complex, transferase/rna/dna
Biological sourceSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Cellular locationNucleus P04050 P08518 P16370 P20433 P20434 P34087 P20436 P38902
Cytoplasm  P20435
Nucleus, nucleolus  P27999 P22139 P40422
Total number of polymer chains15
Total molecular weight553168.16
Barnes, C.O.,Calero, M.,Malik, I.,Saphr, H.,Zhang, Q.,Pullara, F.,Kaplan, C.D.,Calero, G. (deposition date: 2015-06-17, release date: 2015-08-26, Last modification date: 2017-09-27)
Primary citation
Barnes, C.O.,Calero, M.,Malik, I.,Graham, B.W.,Spahr, H.,Lin, G.,Cohen, A.E.,Brown, I.S.,Zhang, Q.,Pullara, F.,Trakselis, M.A.,Kaplan, C.D.,Calero, G.
Crystal Structure of a Transcribing RNA Polymerase II Complex Reveals a Complete Transcription Bubble.
Mol.Cell, 59:258-269, 2015
PubMed: 26186291 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2015.06.034
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRNA backbone0.26353.4%8.5%0.70MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution