Crystal structure of a transcribing RNA Polymerase II complex reveals a complete transcription bubble

Summary for 5C4A

Related5C3E 5C44 5C4J 5C4X
DescriptorDNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB3, ... (17 entities in total)
Functional Keywordsprotein-dna complex, rna polymerase ii, transcribing complex, transcription bubble, transferase-rna-dna complex, transferase/rna/dna
Biological sourceSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Total number of polymer chains15
Total molecular weight553168.16
Barnes, C.O.,Calero, M.,Malik, I.,Saphr, H.,Zhang, Q.,Pullara, F.,Kaplan, C.D.,Calero, G. (deposition date: 2015-06-17, release date: 2015-08-26, Last modification date: 2019-12-25)
Primary citation
Barnes, C.O.,Calero, M.,Malik, I.,Graham, B.W.,Spahr, H.,Lin, G.,Cohen, A.E.,Brown, I.S.,Zhang, Q.,Pullara, F.,Trakselis, M.A.,Kaplan, C.D.,Calero, G.
Crystal Structure of a Transcribing RNA Polymerase II Complex Reveals a Complete Transcription Bubble.
Mol.Cell, 59:258-269, 2015
PubMed: 26186291 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2015.06.034
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRNA backbone 0.2635 3.4% 8.5% 0.70MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
PDB entries from 2020-10-28