5C44
Crystal structure of a transcribing RNA Polymerase II complex reveals a complete transcription bubble
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 220.221, 391.843, 282.312 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.000 - 3.950 |
| R-factor | 0.2161 |
| Rwork | 0.215 |
| R-free | 0.23630 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 195.608 | 90.784 | 4.110 |
| High resolution limit [Å] | 3.852 | 12.330 | 3.852 |
| Rmerge | 0.232 | 0.088 | 0.773 |
| Rpim | 0.050 | 0.512 | |
| Total number of observations | 13462 | 42263 | |
| Number of reflections | 104726 | ||
| <I/σ(I)> | 2.4 | 5.8 | 1 |
| Completeness [%] | 93.9 | 98.1 | 91.1 |
| Redundancy | 3.1 | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | PEG 6000, Ammonium Acetate, Sodium Acetate, DTT |
