5A77

Crystal structure of the homing endonuclease I-CvuI in complex with I- CreI target (C1221) in the presence of 2 mM Mg revealing DNA cleaved

Summary for 5A77

Related5A72 5A73 5A74 5A75 5A78
DescriptorDNA ENDONUCLEASE I-CVUI, 14MER DNA, 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*DCP)-3', 10MER DNA, 5'-D(*GP*AP*CP*GP*TP*TP*TP*TP* GP*AP*DGP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3', ... (5 entities in total)
Functional Keywordshydrolase-dna complex, gene targeting, genetics, protein-dna interaction, homing endonucleases, hydrolase/dna
Biological sourceCHLORELLA VULGARIS
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Cellular locationPlastid, chloroplast P56347
Total number of polymer chains6
Total molecular weight53281.31
Authors
Molina, R.,Redondo, P.,LopezMendez, B.,Villate, M.,Merino, N.,Blanco, F.J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G. (deposition date: 2015-07-03, release date: 2015-09-23, Last modification date: 2015-12-09)
Primary citation
Molina, R.,Redondo, P.,Lopez-Mendez, B.,Villate, M.,Merino, N.,Blanco, F.J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G.
Crystal Structure of the Homing Endonuclease I-Cvui Provides a New Template for Genome Modification
J.Biol.Chem., 290:28727-, 2015
PubMed: 26363068 (PDB entries with the same primary citation)
DOI: 10.1074/JBC.M115.678342
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.5 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRSRZ outliers803.6%4.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution