5A72

Crystal structure of the homing endonuclease I-CvuI in complex with its target (Sro1.3) in the presence of 2 mM Ca

Summary for 5A72

Related5A73 5A74 5A75 5A77 5A78
DescriptorDNA ENDONUCLEASE I-CVUI, 24MER DNA, 5'-D(*DTP*CP*AP*GP*AP*AP*CP*GP*TP*CP*GP*TP*AP *DCP*GP*AP*CP*GP*TP*TP*CP*TP*GP*A)-3', CALCIUM ION, ... (4 entities in total)
Functional Keywordshydrolase-dna complex, gene targeting, genetics, protein-dna interaction, homing endonucleases, hydrolase/dna
Biological sourceCHLORELLA VULGARIS (GREEN ALGAE)
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Cellular locationPlastid, chloroplast P56347
Total number of polymer chains4
Total molecular weight53376.7
Authors
Molina, R.,Redondo, P.,LopezMendez, B.,Villate, M.,Merino, N.,Blanco, F.J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G. (deposition date: 2015-07-02, release date: 2015-09-23, Last modification date: 2015-12-09)
Primary citation
Molina, R.,Redondo, P.,Lopez-Mendez, B.,Villate, M.,Merino, N.,Blanco, F.J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G.
Crystal Structure of the Homing Endonuclease I-Cvui Provides a New Template for Genome Modification
J.Biol.Chem., 290:28727-, 2015
PubMed: 26363068 (PDB entries with the same primary citation)
DOI: 10.1074/JBC.M115.678342
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.6 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRSRZ outliers501.8%0.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5a72
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Molmil generated image of 5a72
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Molmil generated image of 5a72
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