5A72

Crystal structure of the homing endonuclease I-CvuI in complex with its target (Sro1.3) in the presence of 2 mM Ca

Summary for 5A72

• Entry DOI: 10.2210/pdb5a72/pdb
• Related: 5A73 5A74 5A75 5A77 5A78
• Descriptor: DNA ENDONUCLEASE I-CVUI, 24MER DNA, 5'-D(*DTP*CP*AP*GP*AP*AP*CP*GP*TP*CP*GP*TP*AP *DCP*GP*AP*CP*GP*TP*TP*CP*TP*GP*A)-3', CALCIUM ION, ... (4 entities in total)
• Functional Keywords: hydrolase-dna complex, gene targeting, genetics, protein-dna interaction, homing endonucleases, hydrolase/dna
• Biological source: CHLORELLA VULGARIS (GREEN ALGAE); More
• Cellular location: Plastid, chloroplast: P56347
• Total number of polymer chains: 4
• Total formula weight: 53376.70

Authors

Molina, R.,Redondo, P.,LopezMendez, B.,Villate, M.,Merino, N.,Blanco, F.J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G. (deposition date: 2015-07-02, release date: 2015-09-23, Last modification date: 2024-01-10)

Primary citation

Molina, R.,Redondo, P.,Lopez-Mendez, B.,Villate, M.,Merino, N.,Blanco, F.J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G.
Crystal Structure of the Homing Endonuclease I-Cvui Provides a New Template for Genome Modification
J.Biol.Chem., 290:28727-, 2015

PubMed Abstract: Homing endonucleases recognize and generate a DNA double-strand break, which has been used to promote gene targeting. These enzymes recognize long DNA stretches; they are highly sequence-specific enzymes and display a very low frequency of cleavage even in complete genomes. Although a large number of homing endonucleases have been identified, the landscape of possible target sequences is still very limited to cover the complexity of the whole eukaryotic genome. Therefore, the finding and molecular analysis of homing endonucleases identified but not yet characterized may widen the landscape of possible target sequences. The previous characterization of protein-DNA interaction before the engineering of new homing endonucleases is essential for further enzyme modification. Here we report the crystal structure of I-CvuI in complex with its target DNA and with the target DNA of I-CreI, a homologue enzyme widely used in genome engineering. To characterize the enzyme cleavage mechanism, we have solved the I-CvuI DNA structures in the presence of non-catalytic (Ca(2+)) and catalytic ions (Mg(2+)). We have also analyzed the metal dependence of DNA cleavage using Mg(2+) ions at different concentrations ranging from non-cleavable to cleavable concentrations obtained from in vitro cleavage experiments. The structure of I-CvuI homing endonuclease expands the current repertoire for engineering custom specificities, both by itself as a new scaffold alone and in hybrid constructs with other related homing endonucleases or other DNA-binding protein templates.

PubMed: 26363068
DOI: 10.1074/JBC.M115.678342

Experimental method

X-RAY DIFFRACTION (2.6 Å)