5A72

Crystal structure of the homing endonuclease I-CvuI in complex with its target (Sro1.3) in the presence of 2 mM Ca

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA ENDONUCLEASE I-CVUI	polymer	172	19278.5	2	UniProt (P56347) Pfam (PF00961)	CHLORELLA VULGARIS (GREEN ALGAE)	23S RRNA INTRON PROTEIN, I-CVUI
2	C, D (C, D)	24MER DNA, 5'-D(*DTP*CP*AP*GP*AP*AP*CP*GP*TP*CP*GP*TP*AP *DCP*GP*AP*CP*GP*TP*TP*CP*TP*GP*A)-3'	polymer	24	7369.8	2		SYNTHETIC CONSTRUCT
3	E, F (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	G, H, I, J (A, B, C, D)	water	water		18.0	86	Chemie (HOH)

Sequence modifications

A, B: 3 - 162 (UniProt: P56347)

PDB	External Database	Details
Ala 2	-	expression tag
Ser 163	-	expression tag
Ala 164	-	expression tag
Ala 165	-	expression tag
Leu 166	-	expression tag
Glu 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
Gln 54	Arg 53	conflict
Asn 100	Gln 99	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	53296.5
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	53376.7

*Water molecules are not included.