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5A77

Crystal structure of the homing endonuclease I-CvuI in complex with I- CreI target (C1221) in the presence of 2 mM Mg revealing DNA cleaved

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, BDNA ENDONUCLEASE I-CVUIpolymer17219292.52UniProt (P56347)
Pfam (PF00961)
In PDB
CHLORELLA VULGARIS
2C, D14MER DNA, 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*DCP)-3'polymer144248.82SYNTHETIC CONSTRUCT
3E, F10MER DNA, 5'-D(*GP*AP*CP*GP*TP*TP*TP*TP* GP*AP*DGP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3'polymer103075.02SYNTHETIC CONSTRUCT
4B, DMAGNESIUM IONnon-polymer24.32Chemie (MG)
5waterwater18.017Chemie (HOH)
Sequence modifications
A, B: 3 - 162 (UniProt: P56347)
PDBExternal DatabaseDetails
Ala 2-expression tag
Gln 54Arg 53conflict
Ser 163-expression tag
Ala 164-expression tag
Ala 165-expression tag
Leu 166-expression tag
Glu 167-expression tag
His 168-expression tag
His 169-expression tag
His 170-expression tag
His 171-expression tag
His 172-expression tag
His 173-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains6
Total formula weight53232.7
Non-Polymers*Number of molecules2
Total formula weight48.6
All*Total formula weight53281.3
*Water molecules are not included.

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