5A1U

The structure of the COPI coat triad

Summary for 5A1U

• Entry DOI: 10.2210/pdb5a1u/pdb
• Related: 5A1V 5A1W 5A1X 5A1Y
• EMDB information: 2985
• Descriptor: ADP-RIBOSYLATION FACTOR 1, COATOMER SUBUNIT ALPHA, COATOMER SUBUNIT BETA', ... (7 entities in total)
• Functional Keywords: transport protein, structural protein, copi, coatomer, coated vesicles
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); More
• Cellular location: Golgi apparatus: P11076; Cytoplasm . Xenin: Secreted : Q8CIE6; Cytoplasm, cytosol : O55029 Q9QZE5; Cytoplasm : P61924 Q9JIF7 Q5XJY5
• Total number of polymer chains: 8
• Total formula weight: 570496.93

Authors

Dodonova, S.O.,Diestelkoetter-Bachert, P.,von Appen, A.,Hagen, W.J.H.,Beck, R.,Beck, M.,Wieland, F.,Briggs, J.A.G. (deposition date: 2015-05-06, release date: 2015-07-08, Last modification date: 2024-05-08)

Primary citation

Dodonova, S.O.,Diestelkoetter-Bachert, P.,Von Appen, A.,Hagen, W.J.H.,Beck, R.,Beck, M.,Wieland, F.,Briggs, J.A.G.
Vesicular Transport. A Structure of the Copi Coat and the Role of Coat Proteins in Membrane Vesicle Assembly.
Science, 349:195-, 2015

PubMed Abstract: Transport of material within cells is mediated by trafficking vesicles that bud from one cellular compartment and fuse with another. Formation of a trafficking vesicle is driven by membrane coats that localize cargo and polymerize into cages to bend the membrane. Although extensive structural information is available for components of these coats, the heterogeneity of trafficking vesicles has prevented an understanding of how complete membrane coats assemble on the membrane. We combined cryo-electron tomography, subtomogram averaging, and cross-linking mass spectrometry to derive a complete model of the assembled coat protein complex I (COPI) coat involved in traffic between the Golgi and the endoplasmic reticulum. The highly interconnected COPI coat structure contradicted the current "adaptor-and-cage" understanding of coated vesicle formation.

PubMed: 26160949
DOI: 10.1126/SCIENCE.AAB1121

Experimental method

ELECTRON MICROSCOPY (13 Å)