5MAR
Structure of human SIRT2 in complex with 1,2,4-Oxadiazole inhibitor and ADP ribose.
Summary for 5MAR
| Entry DOI | 10.2210/pdb5mar/pdb |
| Descriptor | NAD-dependent protein deacetylase sirtuin-2, ZINC ION, [(2R,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL [HYDROXY-[[(2R,3S,4R,5S)-3,4,5-TRIHYDROXYOXOLAN-2-YL]METHOXY]PHOSPHORYL] HYDROGEN PHOSPHATE, ... (9 entities in total) |
| Functional Keywords | sirtuin, nad-dependent protein deacylase, inhibitor complex, hydrolase |
| Biological source | Homo sapiens (Human) |
| Cellular location | Nucleus. Isoform 1: Cytoplasm . Isoform 2: Cytoplasm . Isoform 5: Cytoplasm : Q8IXJ6 |
| Total number of polymer chains | 2 |
| Total formula weight | 71700.89 |
| Authors | Moniot, S.,Steegborn, C. (deposition date: 2016-11-04, release date: 2017-03-15, Last modification date: 2024-01-17) |
| Primary citation | Moniot, S.,Forgione, M.,Lucidi, A.,Hailu, G.S.,Nebbioso, A.,Carafa, V.,Baratta, F.,Altucci, L.,Giacche, N.,Passeri, D.,Pellicciari, R.,Mai, A.,Steegborn, C.,Rotili, D. Development of 1,2,4-Oxadiazoles as Potent and Selective Inhibitors of the Human Deacetylase Sirtuin 2: Structure-Activity Relationship, X-ray Crystal Structure, and Anticancer Activity. J. Med. Chem., 60:2344-2360, 2017 Cited by PubMed Abstract: Sirt2 is a target for the treatment of neurological, metabolic, and age-related diseases including cancer. Here we report a series of Sirt2 inhibitors based on the 1,2,4-oxadiazole scaffold. These compounds are potent Sirt2 inhibitors active at single-digit μM level by using the Sirt2 substrate α-tubulin-acetylLys40 peptide and inactive up to 100 μM against Sirt1, -3, and -5 (deacetylase and desuccinylase activities). Their mechanism of inhibition is uncompetitive toward both the peptide substrate and NAD, and the crystal structure of a 1,2,4-oxadiazole analog in complex with Sirt2 and ADP-ribose reveals its orientation in a still unexplored subcavity useful for further inhibitor development. Tested in leukemia cell lines, 35 and 39 induced apoptosis and/or showed antiproliferative effects at 10 or 25 μM after 48 h. Western blot analyses confirmed the involvement of Sirt2 inhibition for their effects in NB4 and in U937 cells. Our results provide novel Sirt2 inhibitors with a compact scaffold and structural insights for further inhibitor improvement. PubMed: 28240897DOI: 10.1021/acs.jmedchem.6b01609 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.89 Å) |
Structure validation
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