4ZP4

Crystal Structure of the Heterodimeric HIF-2a:ARNT Complex

Summary for 4ZP4

• Entry DOI: 10.2210/pdb4zp4/pdb
• Related: 4ZPH 4ZPK 4ZPR
• Descriptor: Aryl hydrocarbon receptor nuclear translocator, Endothelial PAS domain-containing protein 1 (3 entities in total)
• Functional Keywords: arnt, hif-2a complex, bhlh-pas, protein transport-transcription complex, protein transport/transcription
• Biological source: Mus musculus (Mouse); More
• Cellular location: Nucleus: P53762 P97481
• Total number of polymer chains: 4
• Total formula weight: 169276.69

Authors

Wu, D.,Potluri, N.,Lu, J.,Kim, Y.,Rastinejad, F. (deposition date: 2015-05-07, release date: 2015-08-12, Last modification date: 2023-09-27)

Primary citation

Wu, D.,Potluri, N.,Lu, J.,Kim, Y.,Rastinejad, F.
Structural integration in hypoxia-inducible factors.
Nature, 524:303-308, 2015

PubMed Abstract: The hypoxia-inducible factors (HIFs) coordinate cellular adaptations to low oxygen stress by regulating transcriptional programs in erythropoiesis, angiogenesis and metabolism. These programs promote the growth and progression of many tumours, making HIFs attractive anticancer targets. Transcriptionally active HIFs consist of HIF-α and ARNT (also called HIF-1β) subunits. Here we describe crystal structures for each of mouse HIF-2α-ARNT and HIF-1α-ARNT heterodimers in states that include bound small molecules and their hypoxia response element. A highly integrated quaternary architecture is shared by HIF-2α-ARNT and HIF-1α-ARNT, wherein ARNT spirals around the outside of each HIF-α subunit. Five distinct pockets are observed that permit small-molecule binding, including PAS domain encapsulated sites and an interfacial cavity formed through subunit heterodimerization. The DNA-reading head rotates, extends and cooperates with a distal PAS domain to bind hypoxia response elements. HIF-α mutations linked to human cancers map to sensitive sites that establish DNA binding and the stability of PAS domains and pockets.

PubMed: 26245371
DOI: 10.1038/nature14883

Experimental method

X-RAY DIFFRACTION (2.355 Å)