4ZK0

Psoriasis pathogenesis - Pso p27 constitute a compact structure forming large aggregates. High pH structure

Summary for 4ZK0

• Entry DOI: 10.2210/pdb4zk0/pdb
• Descriptor: Serpin B4, ZINC ION (3 entities in total)
• Functional Keywords: immune system, hydrolase inhibitor, autoimmunity, pathogenesis, pso p27, pso p27-complex, psoriasis, scca1, serpinb3
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm : P29508
• Total number of polymer chains: 1
• Total formula weight: 44751.27

Authors

Helland, R.,Lysvand, H.,Slupphaug, G.,Iversen, O.J. (deposition date: 2015-04-29, release date: 2015-07-01, Last modification date: 2024-01-10)

Primary citation

Lysvand, H.,Helland, R.,Hagen, L.,Slupphaug, G.,Iversen, O.J.
Psoriasis pathogenesis - Pso p27 constitutes a compact structure forming large aggregates.
Biochem Biophys Rep, 2:132-136, 2015

PubMed Abstract: Psoriasis is a chronic inflammatory skin disease. The absence of microbial organisms as potential causal agents has given rise to the hypothesis that the inflammation is due to an autoimmune reaction. The defined inflamed areas of the skin lesions argue for an immunological disease with a local production of a causal antigen. Pso p27 is a protein generated in mast cells in psoriatic plaques, but not in uninvolved skin. We recently demonstrated that the Pso p27 is generated by cleavage of SerpinB3 (SCCA1) in the presence of mast cell associated chymase. In this communication we demonstrate by X-ray crystallographic analysis that the cleavage products associate into a complex similar to SCCA1, but with the reactive centre loop inserted into a 5-stranded central β-sheet. Native gel electrophoresis show that these Pso p27 complexes form large aggregates which may be of significance with respect to an immunogenic role of Pso p27.

PubMed: 29124154
DOI: 10.1016/j.bbrep.2015.06.001

Experimental method

X-RAY DIFFRACTION (2.15 Å)