4Z3S

Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic A201A, mRNA and three tRNAs in the A, P and E sites at 2.65A resolution

This is a large structure.

Summary for 4Z3S

Related4Z3R 4Z3Q
Descriptor23S Ribosomal RNA, 5S Ribosomal RNA, 50S ribosomal protein L2, ... (61 entities in total)
Functional Keywordshygromycin a, a201a, antibiotic, 70s ribosome, inhibition of translation, peptidyl transferase inhibitors, trna accommodation intermediate, single-molecule fret, ribosome-antibiotic complex, ribosome/antibiotic
Biological sourceEscherichia coli
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Total number of polymer chains112
Total molecular weight4562010.41
Authors
Polikanov, Y.S.,Starosta, A.L.,Juette, M.F.,Altman, R.B.,Terry, D.S.,Lu, W.,Burnett, B.J.,Dinos, G.,Reynolds, K.,Blanchard, S.C.,Steitz, T.A.,Wilson, D.N. (deposition date: 2015-03-31, release date: 2015-06-03, Last modification date: 2017-09-27)
Primary citation
Polikanov, Y.S.,Starosta, A.L.,Juette, M.F.,Altman, R.B.,Terry, D.S.,Lu, W.,Burnett, B.J.,Dinos, G.,Reynolds, K.A.,Blanchard, S.C.,Steitz, T.A.,Wilson, D.N.
Distinct tRNA Accommodation Intermediates Observed on the Ribosome with the Antibiotics Hygromycin A and A201A.
Mol.Cell, 58:832-844, 2015
PubMed: 26028538 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2015.04.014
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.65 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.27190.9%3.1%6.1%0.55MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4z3s
no rotation
Molmil generated image of 4z3s
rotated about x axis by 90°
Molmil generated image of 4z3s
rotated about y axis by 90°
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