4Z3S
Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic A201A, mRNA and three tRNAs in the A, P and E sites at 2.65A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97900 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 208.890, 446.220, 619.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 362.069 - 2.650 |
R-factor | 0.228 |
Rwork | 0.226 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4y4p |
RMSD bond length | 0.005 |
RMSD bond angle | 0.945 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 362.069 | 2.720 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.150 | 1.203 |
Number of reflections | 1628435 | |
<I/σ(I)> | 6.34 | 1.03 |
Completeness [%] | 98.5 | 99.3 |
Redundancy | 3.469 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |