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4Z3S

Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic A201A, mRNA and three tRNAs in the A, P and E sites at 2.65A resolution

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyPIXEL
Collection date2014-10-11
DetectorDECTRIS PILATUS 6M-F
Wavelength(s)0.97900
Spacegroup nameP 21 21 21
Unit cell lengths208.890, 446.220, 619.480
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution362.069 - 2.650
R-factor0.228
Rwork0.226
R-free0.27100
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4y4p
RMSD bond length0.005
RMSD bond angle0.945
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]362.0692.720
High resolution limit [Å]2.6502.650
Rmerge0.1501.203
Number of reflections1628435
<I/σ(I)>6.341.03
Completeness [%]98.599.3
Redundancy3.469
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION7.62920.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME

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