Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to mRNA and A-, P- and E-site tRNAs at 2.5A resolution

This is a large structure.

Summary for 4Y4P

Descriptor23S Ribosomal RNA, 5S Ribosomal RNA, 50S ribosomal protein L2, ... (60 entities in total)
Functional Keywordsribosome, rrna modofications, pseudouridine, rrna methylation, ribosomal protein post-translational modifications, iron-sulfur cluster, beta-thio-aspartate
Biological sourceEscherichia coli
Total number of polymer chains112
Total molecular weight4571403.42
Polikanov, Y.S.,Melnikov, S.V.,Soll, D.,Steitz, T.A. (deposition date: 2015-02-10, release date: 2015-03-18, Last modification date: 2017-09-27)
Primary citation
Polikanov, Y.S.,Melnikov, S.V.,Soll, D.,Steitz, T.A.
Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly.
Nat.Struct.Mol.Biol., 22:342-344, 2015
PubMed: 25775268 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb.2992
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.281100.9%3.2%7.7%0.53MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4y4p
no rotation
Molmil generated image of 4y4p
rotated about x axis by 90°
Molmil generated image of 4y4p
rotated about y axis by 90°
PDBj@FacebookPDBj@TwitterwwPDBwwPDB Foundation

Copyright © 2013-2019 Protein Data Bank Japan