4Y4P

Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to mRNA and A-, P- and E-site tRNAs at 2.5A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97900
Spacegroup name	P 21 21 21
Unit cell lengths	209.700, 450.050, 624.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	122.010 - 2.500
R-factor	0.2332
Rwork	0.231
R-free	0.28070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.063
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	312.045		2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.141	0.024	1.116
Rmeas	0.168	0.029	1.418
Total number of observations	6441594
Number of reflections	1962676	22669	130706
<I/σ(I)>	8.27	29.91	0.9
Completeness [%]	97.9	95.9	88.5
Redundancy	3.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME