4YC4

Crystal structure of phosphatidyl inositol 4-kinase II alpha in complex with nucleotide analog

Summary for 4YC4

• Entry DOI: 10.2210/pdb4yc4/pdb
• Related: 4PLA
• Descriptor: Phosphatidylinositol 4-kinase type 2-alpha,Lysozyme,Phosphatidylinositol 4-kinase type 2-alpha, [(1S,3S,4S)-3-(6-amino-9H-purin-9-yl)bicyclo[2.2.1]hept-1-yl]methanol, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: kinase, complex, inhibitor, transferase
• Biological source: Homo sapiens (Human); More
• Cellular location: Golgi apparatus, trans-Golgi network membrane ; Lipid-anchor : Q9BTU6
• Total number of polymer chains: 1
• Total formula weight: 63927.42

Authors

Klima, M.,Boura, E. (deposition date: 2015-02-19, release date: 2015-07-15, Last modification date: 2024-05-08)

Primary citation

Klima, M.,Baumlova, A.,Chalupska, D.,Hrebabecky, H.,Dejmek, M.,Nencka, R.,Boura, E.
The high-resolution crystal structure of phosphatidylinositol 4-kinase II beta and the crystal structure of phosphatidylinositol 4-kinase II alpha containing a nucleoside analogue provide a structural basis for isoform-specific inhibitor design.
Acta Crystallogr.,Sect.D, 71:1555-1563, 2015

PubMed Abstract: Phosphatidylinositol 4-phosphate (PI4P) is the most abundant monophosphoinositide in eukaryotic cells. Humans have four phosphatidylinositol 4-kinases (PI4Ks) that synthesize PI4P, among which are PI4K IIβ and PI4K IIα. In this study, two crystal structures are presented: the structure of human PI4K IIβ and the structure of PI4K IIα containing a nucleoside analogue. The former, a complex with ATP, is the first high-resolution (1.9 Å) structure of a PI4K. These structures reveal new details such as high conformational heterogeneity of the lateral hydrophobic pocket of the C-lobe and together provide a structural basis for isoform-specific inhibitor design.

PubMed: 26143926
DOI: 10.1107/S1399004715009505

Experimental method

X-RAY DIFFRACTION (2.58 Å)