4YC4
Crystal structure of phosphatidyl inositol 4-kinase II alpha in complex with nucleotide analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 79.414, 103.770, 78.109 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.068 - 2.580 |
| R-factor | 0.2162 |
| Rwork | 0.214 |
| R-free | 0.25580 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.070 | 2.670 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.071 | 0.404 |
| Number of reflections | 20124 | |
| <I/σ(I)> | 15.98 | 2.36 |
| Completeness [%] | 95.8 | 74.15 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM HEPES pH = 7.0, 10% w/v PEG 4000, 10% v/v 2-propanol |
