4XR0

Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- G/T mismatch.

Summary for 4XR0

• Entry DOI: 10.2210/pdb4xr0/pdb
• Related: 1ECR 2EWJ 2I05 2I06 4XR1 4XR2 4XR3
• Descriptor: DNA replication terminus site-binding protein, DNA (5'-D(*AP*GP*TP*TP*AP*CP*AP*AP*CP*AP*TP*AP*GP*T)-3'), DNA (5'-D(*AP*TP*TP*AP*TP*GP*TP*TP*GP*TP*AP*AP*CP*TP*A)-3'), ... (6 entities in total)
• Functional Keywords: dna complex, replication, tus, ter, replication-dna complex, replication/dna
• Biological source: Escherichia coli (strain K12); More
• Total number of polymer chains: 3
• Total formula weight: 47354.38

Authors

Oakley, A.J. (deposition date: 2015-01-20, release date: 2015-08-26, Last modification date: 2023-09-27)

Primary citation

Elshenawy, M.M.,Jergic, S.,Xu, Z.Q.,Sobhy, M.A.,Takahashi, M.,Oakley, A.J.,Dixon, N.E.,Hamdan, S.M.
Replisome speed determines the efficiency of the Tus-Ter replication termination barrier.
Nature, 525:394-398, 2015

PubMed Abstract: In all domains of life, DNA synthesis occurs bidirectionally from replication origins. Despite variable rates of replication fork progression, fork convergence often occurs at specific sites. Escherichia coli sets a 'replication fork trap' that allows the first arriving fork to enter but not to leave the terminus region. The trap is set by oppositely oriented Tus-bound Ter sites that block forks on approach from only one direction. However, the efficiency of fork blockage by Tus-Ter does not exceed 50% in vivo despite its apparent ability to almost permanently arrest replication forks in vitro. Here we use data from single-molecule DNA replication assays and structural studies to show that both polarity and fork-arrest efficiency are determined by a competition between rates of Tus displacement and rearrangement of Tus-Ter interactions that leads to blockage of slower moving replisomes by two distinct mechanisms. To our knowledge this is the first example where intrinsic differences in rates of individual replisomes have different biological outcomes.

PubMed: 26322585
DOI: 10.1038/nature14866

Experimental method

X-RAY DIFFRACTION (2.8 Å)