1ECR

ESCHERICHIA COLI REPLICATION TERMINATOR PROTEIN (TUS) COMPLEXED WITH DNA

Summary for 1ECR

• Entry DOI: 10.2210/pdb1ecr/pdb
• Descriptor: DNA (5'-D(*TP*TP*AP*GP*TP*TP*AP*CP*AP*AP*CP*AP*TP*AP*CP*T)-3, DNA (5'-D(*TP*AP*GP*TP*AP*TP*GP*TP*TP*GP*TP*AP*AP*CP*TP*A)-3, PROTEIN (REPLICATION-TERMINATOR PROTEIN), ... (4 entities in total)
• Functional Keywords: dna-binding, dna replication, complex (dna-binding protein-dna), replication-dna complex, replication/dna
• Biological source: Escherichia coli
• Total number of polymer chains: 3
• Total formula weight: 45626.60

Authors

Kamada, K.,Morikawa, K. (deposition date: 1996-09-01, release date: 1997-09-05, Last modification date: 2024-02-07)

Primary citation

Kamada, K.,Horiuchi, T.,Ohsumi, K.,Shimamoto, N.,Morikawa, K.
Structure of a replication-terminator protein complexed with DNA.
Nature, 383:598-603, 1996

PubMed Abstract: The crystal structure of the Escherichia coli replication-terminator protein (Tus) bound to terminus-site (Ter) DNA has been determined at 2.7 A resolution. The Tus protein folds into a previously undescribed architecture divided into two domains by a central basic cleft. This cleft accommodates locally deformed B-form Ter DNA and makes extensive contacts with the major groove, mainly through two interdomain beta-strands. The unusual structural features of this complex may explain how the replication fork is halted in only one direction.

PubMed: 8857533
DOI: 10.1038/383598a0

Experimental method

X-RAY DIFFRACTION (2.7 Å)