4XR0
Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- G/T mismatch.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-17 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 64.523, 64.523, 248.335 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.450 - 2.800 |
R-factor | 0.22148 |
Rwork | 0.218 |
R-free | 0.29562 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i06 |
RMSD bond length | 0.016 |
RMSD bond angle | 2.160 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.111 | 0.740 |
Number of reflections | 13845 | |
<I/σ(I)> | 39.5 | 6.6 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 22.1 | 18.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 8-12% (v/v) PEG 3350, 100 mM NaI, 50 mM Bis-Tris |