4XFT

Structure of IL-18 SER Mutant III

Summary for 4XFT

• Entry DOI: 10.2210/pdb4xft/pdb
• Descriptor: Interleukin-18, DIMETHYL SULFOXIDE (3 entities in total)
• Functional Keywords: interleukin-18, il-18, surface entropy reduction, immune defense, cytokine
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 2
• Total formula weight: 36203.08

Authors

Krumm, B.E.,Meng, X.,Xiang, Y.,Deng, J. (deposition date: 2014-12-29, release date: 2015-06-10, Last modification date: 2024-10-23)

Primary citation

Krumm, B.,Meng, X.,Xiang, Y.,Deng, J.
Crystallization of interleukin-18 for structure-based inhibitor design.
Acta Crystallogr.,Sect.F, 71:710-717, 2015

PubMed Abstract: Interleukin-18 (IL-18) is a pleiotropic pro-inflammatory cytokine belonging to the IL-1 superfamily. IL-18 plays an important role in host innate and acquired immune defense, with its activity being modulated in vivo by its naturally occurring antagonist IL-18 binding protein (IL-18BP). Recent crystal structures of human IL-18 (hIL-18) in complex with its antagonist or cognate receptor(s) have revealed a conserved binding interface on hIL-18 representing a promising drug target. An important step in this process is obtaining crystals of apo hIL-18 or hIL-18 in complex with small-molecule inhibitors, preferably under low ionic strength conditions. In this study, surface-entropy reduction (SER) and rational protein design were employed to facilitate the crystallization of hIL-18. The results provide an excellent platform for structure-based drug design.

PubMed: 26057800
DOI: 10.1107/S2053230X15006871

Experimental method

X-RAY DIFFRACTION (2 Å)