4XFT
Structure of IL-18 SER Mutant III
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0,.97929 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.514, 55.042, 113.003 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.17899 |
Rwork | 0.177 |
R-free | 0.21802 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f62 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.295 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.562 | |
Number of reflections | 24057 | |
<I/σ(I)> | 30 | 3.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 35% PEG 3350, 0.1M MES, 5% DMSO |