4WT8

Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex

Summary for 4WT8

Entry DOI	10.2210/pdb4wt8/pdb
Descriptor	mRNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (67 entities in total)
Functional Keywords	ribosome
Biological source	Thermus thermophilus More
Total number of polymer chains	117
Total formula weight	4460697.04
Authors	Amunts, A.,Fiedorczuk, K.,Ramakrishnan, V. (deposition date: 2014-10-29, release date: 2015-01-21, Last modification date: 2024-11-20)
Primary citation	Amunts, A.,Fiedorczuk, K.,Truong, T.T.,Chandler, J.,Peter Greenberg, E.,Ramakrishnan, V. Bactobolin A Binds to a Site on the 70S Ribosome Distinct from Previously Seen Antibiotics. J.Mol.Biol., 427:753-755, 2015 Cited by PubMed Abstract: The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-Å-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations. PubMed: 25562208 DOI: 10.1016/j.jmb.2014.12.018 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.4 Å)

Structure validation