4WT8
Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2013-06-14 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 214.210, 457.450, 639.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.908 - 3.400 |
R-factor | 0.2062 |
Rwork | 0.204 |
R-free | 0.24120 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.615 |
Data scaling software | SCALA (3.3.21) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.908 | 49.908 | 3.580 |
High resolution limit [Å] | 3.400 | 10.750 | 3.400 |
Rmerge | 0.081 | 1.302 | |
Rmeas | 0.298 | ||
Rpim | 0.112 | 0.028 | 0.608 |
Total number of observations | 5690971 | 238813 | 610257 |
Number of reflections | 841002 | ||
<I/σ(I)> | 8.6 | 44.8 | 1.4 |
Completeness [%] | 98.8 | 96.3 | 97.9 |
Redundancy | 6.8 | 8.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5- 4.5%(w/v) PEG550MME |